Figure 5
Myogenic differentiation regulated mRNA expression and myosin protein expression analysis after exposure with NO. (a) myoD, (b) MHC, and (c) myogenin gene expression analysis after exposure with NO (200 of O2 flow rate) for 3 min with and without the presence of L-NNA on day 2, day 4, and day 8. The relative value of mRNA expression of these genes were measured by real-time RT-PCR. β-actin was used as a reference gene. All values are expressed as ±SD in triplicate. Students t-test was performed as the control (* denotes P < 0.05 and ** denotes P < 0.01). (d) Western blot analysis of the myosin on 8th day after exposure with NO (200 of O2 flow rate) for 3 min. β-tublin was used as the loading control.
