Figure 1
Epileptiform HFHA EEG correlated to seizure behavior dependent change of membrane KCC2 in CTZ-induced seizure animals. (A–E) Representative traces of EEG recordings from rats with different Racine score seizure behaviors induced by i.c.v CTZ injection. DMSO served as vehicle control. No abnormal EEG activity was observed before injection (A) or after DMSO injection (B). CTZ-induced either single low frequency spike discharge (C) along with MRS I-II seizure behaviors (Supplementary video 1a), or high frequency and high amplitude (HFHA) bursting activities (D) associated with Racine III (Supplementary video 1b), or HFHA bursting activities (E) associated with Racine IV-VII seizure behaviors (Supplementary video 1c). (F) Sample power spectrum analysis of the representative EEG recordings from (A–E), and the mean peak power frequency analysis associated with MRS I-II, III or IV-VII behaviors. (G) Representative cropped western blot results showing the quantity of membrane KCC2 in different seizure level animals. The gels were run under the same experimental conditions and were cropped around 140 and 46 KDa. The full-length gels were presented in Supplementary Fig. 2. Bar histogram showing quantification of KCC2 expression in different seizure level animal groups. CTZ sub-groups were all normalized to DMSO group. KCC2 was significantly downregulated in Racine III (***P < 0.001) and IV-VII score animals (***P < 0.001).
