Figure 5 | Scientific Reports

Figure 5

From: Brain-derived neurotrophic factor promotes human granulosa-like tumor cell steroidogenesis and proliferation by activating the FSH receptor-mediated signaling pathway

Figure 5

Co-localization of FSH receptor and phosphorylated protein. (A) After treatment, cells were fixed and subjected to immunofluorescence. Anti-FSHR antibody was probed with anti-rabbit IgG H&L (FITC) (green); antibody against serine, threonine, and tyrosine phosphorylated proteins was probed with anti-mouse IgG H&L (TRITC) (red). Cells were counterstained with Hoechst (blue). Co-localization of FSHR and serine, threonine, and tyrosine phosphorylated proteins was shown in merged images (yellow). (B) Quantification of FSHR staining in control and treated cells. (C) Quantification of phosphorylated staining in control and treated cells. *P < 0.05 versus control group. Scale bar = 500 μm.

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