Figure 6

Changes of FSHR phosphorylation in treated KGN cells. (A) Cell extracts were immunoprecipitated with anti-phosphoserine/threonine/tyrosine protein antibody and immunoblots were probed with anti-FSHR antibody to detect phosphorylated FSHR. The “input” panel shows target proteins prior to immunoprecipitation in the extracts. (B,C) Quantitative analysis showing the levels of the FSHR protein and phosphorylated protein in immune complexes. Protein level is expressed as fold change over control; data are mean ± SD. *P < 0.05 and **P < 0.01 versus control group.