Figure 7

Changes of FSHR ubiquitination in treated KGN cells. (A) Cell extracts were immunoprecipitated with anti-ubiquitin antibody, and immunoblots were probed with anti-FSHR antibody to detect ubiquitinated FSHR. The “input” panel shows target proteins prior to immunoprecipitation in the extracts. (B,C) Quantitative analysis showing the levels of FSHR protein and ubiquitinated protein in immune complexes. Protein level is expressed as fold changes over control; data are mean ± SD. *P < 0.05 versus control group.