Figure 8 | Scientific Reports

Figure 8

From: Brain-derived neurotrophic factor promotes human granulosa-like tumor cell steroidogenesis and proliferation by activating the FSH receptor-mediated signaling pathway

Figure 8

Effects of BDNF on cAMP level, PKA and CREB activities, and CREB mRNA expression. KGN cells were treated with FSH (100 ng/ml), BDNF (5 ng/ml) and FSH (100 ng/ml) plus BDNF (5 ng/ml), respectively, for 24 h. (A and B) Cell extracts were subjected to ELISA for the determination of cAMP production and PKA kinase activity in treated and untreated cells. (C) Western blot analysis of protein extracts from treated and untreated cell showing the levels of pCREB (Ser133) and total CREB. (D) Relative CREB activity was derived as pCREB normalized to total CREB. (E) Total cellular RNA was extracted, and CREB mRNA levels were determined by real-time PCR. Relative gene expression levels were normalized to β-actin, and expressed as fold change. *P < 0.05, **P < 0.01 and ***P < 0.001 versus control group.

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