Figure 9

Effects of BDNF siRNA and FSH treatment on estradiol secretion, and FSHR, aromatase and phosphorylated CREB levels. (A) Production and secretion of estradiol was detected by chemiluminescent immunoassay system. (B) Western blotting measured the protein levels of BDNF, FSHR, aromatase and pCREB (Ser133) in KGN cells transfected with BDNF siRNA. (C) Relative protein levels in (B) are expressed as fold changes over control. *P < 0.05, **P < 0.01 and ***P < 0.001 versus control group.