Figure 1
Approach for the detection of FAM134B protein in colon cancer. Schematic showing the steps involved in the fabrication of electrochemical assay for the detection of FAM134B protein. FAM134B proteins extracted from colon cancer cell lysates and serum samples complexed with surface attached-biotinylated FAM134B antibody which hinders the interfacial electron transfer reaction of [Fe(CN)6]3−/4−. Inset, the DPV current generated at the (i) unmodified and (ii-iv) modified electrodes. Δir is the difference between relative DPV signals obtained at antibody/BSA- and antibody/BSA/antigen-modified electrodes.
