Figure 2
CCG-222740 is more effective than CCG-203971 in rabbit conjunctival fibroblasts. (A,B) Rabbit conjunctival fibroblasts were seeded in collagen type I matrix and cultured for 7 days as in Fig. 1A and B. (C,D) Analysis of ACTA2 gene expression in rabbit conjunctival fibroblasts was analysed by qRT-PCR as in Fig. 1E and F. Results represent mean ± SEM for triplicate experiments. *P < 0.05; **P < 0.01. (E) Rabbit conjunctival fibroblasts were transfected with the SRF reporter 3DA.luc, serum-starved, and treated with different concentrations of CCG-222740 or CCG-203971 or 0.1% DMSO control for 24 hours. Results represent mean ± SEM for triplicate experiments; (F) HEK293 cells were transfected with the SRF reporter SRE.L luc and treated as in (E). Results represent mean ± SEM for triplicate experiments; (G) HEK293 cell viability in the presence of CCG-222740 or CCG-203971 was measured using the WST-1 assay. Results represent mean ± SEM for triplicate experiments.
