Figure 2 | Scientific Reports

Figure 2

From: Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats

Figure 2

Identification of human bone mesenchymal stem cells (HBMSCs). (a–d) Flow cytometry analysis of HBMSCs with CD44, CD73, CD90 and CD105. (e–h) Characteristics of HBMSCs. (e,f) Bone-forming test, results showed that there were Alizarin Red S staning mineralized matrixes formed. (g) Adipocyte-differentiating test showed that lipid vesicles were visualized with Oil Red O. (h) Cartilage-forming test showed that Alcian Blue staining cartilage could be visualized. (i) Flow cytometry analysis of negative marker cocktail. Results showed that CD11b, CD19, CD34, CD43 and HLA-DR were negative in the HBMSCs. (j,k) Identification of GFP-lentivirus transfected HBMSCs. (k) Image of GFP showed that more than 80% cells were GFP positive. (j) Flow cytometry analysis of HBMSCs showed that over 90% cells were positive for GFP.

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