Figure 2 | Scientific Reports

Figure 2

From: Elevated COX-2 Expression Promotes Angiogenesis Through EGFR/p38-MAPK/Sp1-Dependent Signalling in Pancreatic Cancer

Figure 2

Correlated COX-2 and Sp1 expression in PDAC cell lines. (A) Detection of Sp1 and COX-2 expression in PDAC cell lines (BxPC3, CFPAC and AsPC1) and human pancreatic duct epithelial (HPDE) cells. (B) Relative densities of Sp1 and COX-2 expression are presented as Mean ± S.E (n = 3) of the fold change relative to GAPDH. *P < 0.05 and **P < 0.01. (C) Western blot showing COX-2 expression in BxPC3 and CFPAC cells upon Sp1 knockdown. (D) Western blot showing COX-2 expression in AsPC1 cells upon Sp1 overexpression. (E) Western blot showing Sp1 and COX-2 expression in HPDE cells upon Sp1 knockdown and overexpression. (F,G) CCK8 assay toward HPDE cells upon Sp1 knockout and overexpression.

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