Figure 3
Sp1 transcriptionally activates COX-2 expression in PDAC cells. (A) Schematic diagram of luciferase reporter constructs with the wild-type COX-2 promoter sequence (Pcox-2/wt). (B) Schematic diagram of luciferase reporter constructs with the mutant COX-2 promoter sequence (Pcox-2/wt). The COX-2 promoter sequence was between positions −1122 and +27 from the transcriptional start site. (C) Luciferase activity of pCOX2/wt in PDAC cells. (D) Luciferase activity of Pcox-2/mt in PDAC cells. (E,F) ChIP assay conducted on Sp1 knockout and/or overexpression cells that also transfected with Pcox-2/wt using a specific anti-Sp1 antibody.
