Figure 5

EGFR/p38-MAPK signalling functions upstream of Sp1-driven COX-2 induction. (A) The genetic profile of EGFR in PDAC cells as detected by Western blot. (B) p38, Sp1, COX-2 and VEGF expression upon pharmacological inhibition of EGFR with afatinib. (C) The genetic profile of p38 in PDAC cells as detected by Western blot. (D,E) The expression of Sp1, COX-2 and VEGF when p38 was inhibited using pharmacological (SB203580) or siRNA strategies.