Figure 6

Altered patterns of CD33C2/V and LAIR-1 expression on freshly isolated SLE versus normal blood monocytes. (A,B) Decreased CD33C2/CD33V, LAIR-1, CD14, on SLE blood myelomonocytes vs healthy monocytes as revealed by percent positive (C) Decreased LAIR-1, CD33C2/CD33V domains on SLE blood monocytes vs healthy monocytes as revealed by Mean Fluorescence Intensity (MFI). In (A–C) open bars represent normal (NL) monocytes; solid bars represent SLE myelomonocytes. Results represent the mean +/− SEM of pooled flow cytometry data; two-tailed Student’s t test (A–C) was used to determine significance. For (A,B); N = 9 for CD33C2 and CD33V; N = 8 for LAIR-1 and CD14 for both NL and SLE. For (C); N = 3 for both normal and SLE blood. Gate settings for flow analysis excluded debris and dead cells and are described in Supplementary Fig. S4.