Figure 1
From: Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
ERthermAC targets the endoplasmic reticulum in adipocytes, and its intensity is inversely proportional to temperature. (A) ERthermAC (red) co-localises with ER-Tracker Green (green) in WT-1 cells, as evident from the yellow mix colour after superimposition of signals. Scale bar: 10 μm. (B) Chemical structure of ERthermAC. (C) Differentiated WT-1 cells were stained with ERthermAC, fixed with 4% formaldehyde, and imaged at different temperatures ranging between 18 °C and 43 °C. At higher temperatures, cells display lower ERthermAC fluorescence intensity. Scale bar: 20 μm. (D) Calibration curve shows a reversible non-linear relationship between temperature and fluorescence intensity of ERthermAC in fixed WT-1 cells between 18 °C and 43 °C. Temperature sensitivities determined by the linear fit were −1.07%/°C (R2 = 0.76, n = 15 cells) between 18.1 °C and 35.0 °C, and −4.76%/°C (R2 = 0.83, n = 15 cells) between 37 °C and 43 °C.
