Figure 2
From: Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
ERthermAC shows robust temperature changes after isoproterenol stimulation in WT-1 cells. (A) Individual ISO-stimulated WT-1 cells exhibit decreased ERthermAC fluorescence intensity, suggesting a robust increase in temperature. For time-lapse videos, see Supplementary Video 1. Scale bar: 20 µm.(B) Quantitative analysis of ERthermAC fluorescence intensity after ISO and vehicle stimulation: individual WT-1 cells exhibit a rapid decline in relative intensity after ISO stimulation. The reductions in fluorescence intensity occur at different time points in individual cells. In contrast, vehicle stimulation does not affect ERthermAC intensity. Thick black curves correspond to the mean relative intensities in each group. (C) Scatter plot of ERthermAC relative intensity change shows a significant difference in WT-1 cells. Bars show mean ± SD. WT-1 vehicle: n = 31 cells from 2 cultures; WT-1 ISO: n = 41 cells from 2 cultures.
