Figure 3
From: Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
FCCP stimulation resulted in immediate effects in WT-1 cells. (A) ERthermAC intensity drastically decreases upon FCCP stimulation in all cells, without any lag phase, indicating increased intracellular temperature. The thick black curve corresponds to the mean relative intensity; n = 35 cells from 2 cultures. Scale bar: 20 μm. (B) JC-1 staining shows immediate mitochondrial depolarisation after FCCP treatment. The thick black curve corresponds to the mean relative intensity ratio of green (JC-1 monomers) and red (JC-1 aggregates) signals; n = 50 cells from 2 cultures. Scale bar: 20 μm.
