Figure 5

Expression pattern of the unigenes associated with putative alkaloid biosynthesis in D. officinale. (a) Expression pattern of the unigenes associated with putative upstream elements of alkaloid biosynthetic pathway. Full names of enzymes represented by their abbreviated names were showed in Table S7. The average expression level of the enzyme encoding unigenes in various organs is indicated by a heat map. The grids with different colors from green to red show the relative expression levels to maximum RPKM values, from 0 to 100%, respectively. (b) Phylogenetic analysis of all P450 genes with full-length cDNA from D. officinale. Branches in different background colors indicated different subfamilies. (c) Significance analysis of P450 genes in three leaf contained comparisons, including L vs R, L vs S and L vs F. The grids with different shades of blue show the different significant values, from 0 to 0.01.