Figure 3

Characterization of porcine carotid arterial tissue before and after decellularization. Histology of porcine arteries: (A,B,D and E) native arteries and (C and F) decellularized arteries. (A and B) H&E staining of native arteries (magnification; x4 and x10, respectively). (C) H&E staining of decellularized arteries demonstrating complete removal of cellular components (magnification; x10). (D and E) Masson’s Trichrome staining of native arteries showing high cell density embedded in collagens ECM (green fibers) (magnification; 4x and 10x, respectively). (F) Masson’s Trichrome staining of decellularized artery revealing preservation of the collagenous ECM and no cells (magnification; x10). Representative fluorescent microscope images of a circumferential cross section stained with DAPI: (G) Native arteries and (H) decellularized arteries (magnification; x20). SEM micrographs of the luminal surface: (I) native arteries and (J) decellularized arteries, scale bar indicates 200 μm.