Figure 2
FPRs promoted neuronal differentiation of NSCs. (A–F) FPR1 promoted neuronal differentiation of NSCs. (A,B) Neurospheres were dissociated into single cells and cultured in a differentiation medium with 0, 0.5, 1, and 5 µM fMLF for 6 days. The differentiated cells were then identified by immunocytochemistry using antibodies against β-III tubulin, GFAP, and Olig2. The images in (A) are representative of several independent experiments, and the graph in (B) shows the statistical results. (C) Representative immunoblots. (D) Histograms showing relative levels of β-III tubulin and GFAP. (E) Dissociated NSCs were differentiated in an extremely low density (500 cells/cm2), and then the cells were immunostained with β-III tubulin. (F) Quantified analysis of the total length of neurites per cell, the number of primary neurites, and the number of branch points. (G–L) FPR2 promoted neuronal differentiation of NSCs. (G,H) Neurospheres were dissociated into single cells and cultured in a differentiation medium with 0, 0.05, 0.1, and 0.5 µM MMK-1 for 6 days. The images in (G) are representative of several independent experiments, and the graph in (H) shows the statistical results. (I) Representative immunoblots. (J) Histograms showing relative levels of β-III tubulin and GFAP. (K) Representative micrographs of the differentiated cells immunostained with β-III tubulin. (L) Quantified analysis of the total length of neurites per cell, the number of primary neurites, and the number of branch points. Data were presented as mean ± SEM. *P < 0.05; **P < 0.01; N = 4. Scale bar = 20 µm.
