Figure 4 | Scientific Reports

Figure 4

From: Formyl peptide receptors promotes neural differentiation in mouse neural stem cells by ROS generation and regulation of PI3K-AKT signaling

Figure 4

FPRs is expressed in neurosphere-dissociated cells. (A–L) Flow cytometric analyses of expression of FPR1 and FPR2 at the protein level. Neurosphere-dissociated cells doubly stained with C1qR1 or LeX and FPR1 or FPR2 antibody were assayed by flow cytometry. Results are representative of at least three independent experiments. (A) Negative control for LeX/FPR1, (B) LeX/FPR1, (C) negative control for C1qR1/FPR1, (D) C1qR1/FPR1. (E) Negative control for LeX/FPR2, (F) LeX/FPR2, (G) negative control for C1qR1/FPR2, (H) C1qR1/FPR2. (I) Histograms showing the percentages of FPR1 positive cells in total, Lex+, and Lex− cells, (J)FPR1 positive cells in total, C1qR1+, and C1qR1− cells, (K) FPR2 positive cells in total, Lex+, and Lex− cells, (L) FPR2 positive cells in total, C1qR1+, and C1qR1− cells. (M–P) FPR1 and FPR2 mRNA expression of LeX/C1qR1-sorted cells from dissociated neurospheres was evaluated by quantitative reverse transcription PCR. Results are representative of at least three independent experiments. Data were presented as mean ± SEM; *P < 0.05; N = 6.

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