Figure 6

FPRs promoted neuronal differentiation of Lex⁺FPR⁺, C1qR1⁺FPR⁺ or Lex⁺C1qR1⁺FPR⁺ cells from dissociated neurospheres. (A–D) FPR1 promoted neuronal differentiation of Lex⁺FPR1⁺, C1qR1⁺FPR1⁺ or Lex⁺C1qR1⁺FPR1⁺ cells. Cells were sorted using a Becton Dickinson FACS Aria with markers, including Lex, C1qR1 or FPR1. Each cell population was cultured for 4 days with or without preadministering tBOC for 30 min before administering fMLF, before proceeding to culture with neural differentiation medium. The differentiated cells were then identified by immunocytochemistry using antibodies against β-III tubulin and GFAP. The images in (A,B) are representative of several independent experiments, and the graph in (C,D) shows the statistical results. (E–H) FPR2 promoted neuronal differentiation of Lex⁺FPR2⁺, C1qR1⁺FPR2⁺ or Lex⁺C1qR1⁺FPR2⁺ cells. Each cell population was cultured for 4 days with or without preadministering WRW4 for 30 min before administering MMK-1, following to culture with neural differentiation medium. The differentiated cells were then identified by immunocytochemistry using antibodies against β-III tubulin and GFAP. The images in (E,F) are representative of several independent experiments, and the graph in (G,H) shows the statistical results. Data were presented as mean ± SEM; *P < 0.05. N = 6. Scale bar = 20 µm.