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Figure 1

From: Resveratrol sequentially induces replication and oxidative stresses to drive p53-CXCR2 mediated cellular senescence in cancer cells

Figure 1

Resveratrol induces S phase arrest, DDR and cellular senescence. (A) Cell viability determined by MTT assay. U2OS cells were treated with the indicated concentrations of resveratrol (RSV) for 48 h, and cell viability was determined by MTT. **P < 0.01, when compared with control group. (B) RSV induces S-phase arrest of U2OS cells. Cells were treated with different concentration of RSV for 24 h and then cell cycle distribution was measured by FACS analysis. Data are presented as the mean ± SD of values from triplicate experiments. (C) Detection of γ-H2AX by immunofluorescence. Cells were treated with the indicated concentrations of RSV for 24 h, stained with mouse anti-γ-H2AX(Ser139) antibody and goat anti-mouse TRITC-conjugated secondary antibody, then counterstained with DAPI (blue). (D) Western blotting analysis of p-ATM, p-Chk1 protein levels in U2OS cells treated with different concentration of resveratrol for 24 h. (E) Induction of cellular senescence by RSV. U2OS cells were treated without or with different concentration of RSV for 7 days. Cellular senescence was examined by SA-β-gal staining. (F) Cell cycle distribution of U2OS cells after being treated with different concentration of RSV for 5 days.

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