Figure 5 | Scientific Reports

Figure 5

From: Resveratrol sequentially induces replication and oxidative stresses to drive p53-CXCR2 mediated cellular senescence in cancer cells

Figure 5

Upregulation of p53-CXCR2 axis mediates cellular senescence. (A) CXCR2 mRNA was determined using RT-PCR. U2OS cells were treated with different concentration of RSV for 48 h (left) and 25 μM RSV for 12, 24 and 48 h (right). (B) Detection of CXCR2 by flow cytometry using mouse anti-human CD182 antibody. Cells were treated with RSV (25 and 50 μM) for 48 h. (C) p21 and CXCR2 mRNAs were determined using RT-PCR. U2OS cells were treated with 25 μM RSV for the indicated days. (D) The mRNA levels of CXCR2, p21 in shNeg, shp53 and shCXCR2 U2OS cells were measured 48 h after treatment with RSV (50 μM). (E) The cells were treated the same as (D), on day 7, cellular senescence was examined by SA-β-gal staining.

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