Figure 1 | Scientific Reports

Figure 1

From: Egg antigen p40 of Schistosoma japonicum promotes senescence in activated hepatic stellate cells via SKP2/P27 signaling pathway

Figure 1

Inhibitory effect of Sjp40 on HSCs activation of LX-2 is independent on the cell apoptosis. (a) Effect of Sjp40 on the level of α-SMA in LX-2 cells analyzed by immunofluorescence assay (100× magnification). After treatment with Sjp40 for 48 h, the protein level of α-SMA was measured by immunofluorescence, and Hoechst 33342 was used to stain the nucleus. The cells were photographed using fluorescence microscope. (b) Impact of Sjp40 on LX-2 cells apoptosis. The cells were exposed to different concentrations of Sjp40 (5, 10, 20 μg/mL) or Staurosporin (STS) as a positive control, and at the different times (0, 12, 24, 48, 72 h). And then the level of cleaved-caspase-3 was analyzed by Western blot assay. (c) Effects of Sjp40 on the expression of cell cycle regulatory proteins. The protein levels of Rb and P-Rb in LX-2 cells treated with or without Sjp40 were analyzed by Western blot assay. *p < 0.05 compared to control group. Bar: 50 micrometers.

Back to article page