Figure 3 | Scientific Reports

Figure 3

From: Egg antigen p40 of Schistosoma japonicum promotes senescence in activated hepatic stellate cells via SKP2/P27 signaling pathway

Figure 3

Cellular senescence induced by Sjp40 is dependent on P27. (a) LX-2 cells were transfected with Si-P27 or Si-Con and additionally treated with or without Sjp40 for 48 h. The protein levels of SKP2 and P27 were assayed by Western blot and date were expressed as the mean ± SEM of three or more independent trials. *p < 0.05 compared to the control group; & p < 0.05 compared to the Si-con group; $ p < 0.05 compared to the control group; φ p < 0.05 compared to Si-P27 group. δ p > 0.05 compared to the Si-con group. (b) Knockdown of P27 rescued the Sjp40-induced senescence measured by SA-β-Gal assay (original magnification 200×) and quantitative analysis of the percentage of SA-β-Gal positive cells (%) was expressed as the mean ± SEM of three or more independent trials. ***p < 0.001. Bar: 50 micrometers.

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