Figure 2 | Scientific Reports

Figure 2

From: Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer

Figure 2

Subcellular distribution and knock-down efficiency of LINC00958 and LINC01296 in FL3 cells. (a) Percentage of total RNA found in the nuclear and cytoplasmic fraction in FL3 cells determined by RT-qPCR. Left panel: lincRNA-candidates. Right panel: nuclear (MALAT1) and cytoplasmic (GAPDH) markers for fraction purity. (b and c) FL3 cells were transfected with the indicated LNA longRNA GapmeR (50 nM) followed by total (b) or fractionated (c) RNA extraction 48 hours post-transfection. Knock-down-efficiency of lncRNA-candidates was analyzed by RT-qPCR. Relative total RNA expression levels were normalized to GAPDH and compared to Scr. Relative subcellular RNA expression levels were normalized to Scr. Columns represent the average of two independent experiment measured in triplicates. Bars: standard deviation (SD), ns: not significant and *p < 0.05 and **p < 0.01.

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