Figure 5
In vitro pull-down and mass spectrometry analysis identified LINC00958 interacting proteins. (a) Ingenuity Pathway Analysis. The most significantly pathways and biological functions associated with proteins binding biotin-tagged LINC00958. Only proteins demonstrating FCs ≥ 3, when comparing biotin-tagged LINC00958 to controls were included in the analysis (n = 330). Threshold value; p = 0.05. The analyses were based on knowledge from all species, all tissues, and all data sources. (b) Anti-MTDH RIP using FL3 cell lysates. Detection of endogenous LINC00958 was performed using RT-qPCR (upper panel). The RT-qPCR was performed in triplicates. The experiment was repeated twice and the result of one representative experiment ±SD is shown. IP with a c-Myc antibody was included as negative control. Western blotting analysis (lower panel) was used to evaluate IP of MTDH in the analyzed samples. Loading control input samples: β-actin. FT: flow-through and IP: immunoprecipitation.
