Figure 2
From: Regulation of membrane ruffling by polarized STIM1 and ORAI1 in cortactin-rich domains
Immunolocalization of phospho-STIM1 and cortactin (CTTN). C2C12, HeLa, and U2OS cells were cultured for a minimum of 48 h as indicated in Materials and Methods. C2C12 were stimulated with FBS (20%), and HeLa and U2OS were stimulated with 50 ng/ml EGF for 10 min after 8–10 h of FBS-deprivation in RPMI medium. Fixed cells were used for the immunolocalization of phosho-STIM1 at Ser575 (pS575-STIM1) and cortactin (CTTN). Anti-sheep IgG labelled with Alexa Fluor 594 and anti-mouse IgG labelled with Alexa Fluor 488 were used as secondary antibodies for phospho-STIM1 and CTTN respectively. Images are representative of at least 10 independent experiments. Bar = 10 μm.
