Figure 2

Cnr2 is directly regulated by PEA-mediated PPAR-α activation: bioinformatic analysis and chromatin immunoprecipitation assay. (A) A schematic alignment of the human and rat Cnr2. The black boxes correspond to regions of high sequence homology between the two species contaning the putative PPARα/RXR sites indicated as small black rectangles in the lower part below the schematic. The PPARα/RXR matrix used for the analysis is also shown in the upper part. (B) Average data of the relative amount of the PPARα -immunoprecipitated DNA in HEK293 cells transfected with either control (pCDNA3) or murine PPARα and RXR encoding plasmids. Data are from three to six separate experiments and normalized relative to the input DNA, using the 2^−ΔΔCt formula. The inset shows a representative agarose gel electrophoresis of the qPCR products obtained from PAX7-immunoprecipitated DNA for each experimental condition. Data are shown as mean ± SEM (n = 3) of three independent experiments conducted in triplicate. *p value ≤ 0.05 vscontrol condition (pcDNA-transfected cells), obtained using the unpaired T-TEST for statistical analyses.