Figure 8 | Scientific Reports

Figure 8

From: Immune evasion of Borrelia miyamotoi: CbiA, a novel outer surface protein exhibiting complement binding and inactivating properties

Figure 8

Binding of FH by B. garinii transformants. Serum adsorption assays were employed to detect binding of FH to viable spirochetes. Wild-type B. garinii (Bg) G1, B. burgdorferi (Bb) LW2, and transformant G1/pBS_CbiA (5 × 108 cells each) were incubated in NHS-EDTA to prevent complement activation, washed, and then bound proteins were eluted using 0.1 M glycine (pH 2.0). Both the last wash (w) and the eluate (e) fractions obtained from each strain were separated by 12.5% SDS-PAGE and transferred to nitrocellulose. A polyclonal anti-FH antibody was used to detect FH by Western blotting. The full-length version is presented in Supplementary Figure S9.

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