Figure 2 | Scientific Reports

Figure 2

From: Co-expression of BirA with biotin bait achieves in vivo biotinylation of overexpressed stable N-glycosylated sRAGE in transgenic silkworms

Figure 2

Characterization of sRAGE from transgenic silkworms. (A) Stability of sRAGE during storage at 4 °C. Forty days after purification, 10 μg of protein was applied to SDS-PAGE followed by CBB staining. Lanes 1 and 2: sRAGE expressed in E. coli; lanes 3 and 4: sRAGE expressed in MSGs of transgenic silkworms. Lanes 1 and 3: 1 day storage; lanes 2 and 4: 40 days of storage. Arrows indicate the degraded sRAGE. (B) Binding ability of sRAGE from silkworms to different AGEs after 1 year of storage. ⦁: fructose-AGEs; ▴: ribose-AGEs, ◼: glucose-AGEs, ⬧: control BSA (without glycation). Values are means and SEs of three wells from three independent experiments.

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