Figure 3 | Scientific Reports

Figure 3

From: Co-expression of BirA with biotin bait achieves in vivo biotinylation of overexpressed stable N-glycosylated sRAGE in transgenic silkworms

Figure 3

Analysis of glycosylation. (A) Left panel: CBB staining of 12% gels of sRAGE with (+) or without (−) PNGase treatment. Central panel: glycoprotein staining. sRAGE proteins with or without PNGase treatment were separated with SDS-PAGE, followed by blotting to nitrocellulose membranes and glycoprotein staining. Right panel: detection with anti-RAGE antibodies. (B) MALDI-TOF mass spectra of trypsin-digested sRAGEs without (upper panel) and with PNGase F treatment (lower panel). The observed peaks were as follows: m/z 1870.0 for AVSISIIEPGEEGPTAGSR (315–333), m/z 2127.0 for LISEEDLNSAVDHHHHHH (416–433) and HPETGLFTLQSELMVTPAR (180–198), m/z 2243.2 for VLPNGSLFLPAVGIQDEGIFR (78–98 deamidation) and VLASEGQTVAAGEVLLILEAMK (351–372 deamidation), m/z 2283.2 for RHPETGLFTLQSELMVTPAR (179–198, deamidation), m/z 2512.3 for VYQIPGKPEIVDSASELTAGVPNK (117–140), and m/z 3539.5 for GHHHHHHHHHHSSGHIDDDDKHMAQNITAR (0–29, deamidation).

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