Figure 5 | Scientific Reports

Figure 5

From: Co-expression of BirA with biotin bait achieves in vivo biotinylation of overexpressed stable N-glycosylated sRAGE in transgenic silkworms

Figure 5

Sensorgrams of specific binding of serially diluted ligands to immobilized sRAGE on an SA sensor chip. Sequentially diluted ligands (0.25, 0.5, 1, 2, and 4 μM) were injected at the points indicated by arrows. (A) sRAGE expressed in B. mori. (B) sRAGE expressed in E. coli. Ligand (F-AGEs) diluted with running buffer (PBS) was injected over flow cells at a flow rate of 30 μL/min to allow 1 min association, and the flow cell was allowed 2 min for dissociation using a single-cycle kinetics program. Two reference curves (one curve derived from the injection of buffer only, and one curve derived from nonimmobilized sRAGE) were subtracted from the binding curve to exclude the effects of nonspecific binding. The dissociation constant of ligands to sRAGE was calculated based on the 1:1 (Langmuir) binding model.

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