Figure 4 | Scientific Reports

Figure 4

From: MiR-2425-5p targets RAD9A and MYOG to regulate the proliferation and differentiation of bovine skeletal muscle-derived satellite cells

Figure 4

MiR-2425-5p regulates RAD9A and MYOG expression. (A) MiR-2425-5p binding to the 3′-UTR of RAD9A and MYOG was examined with luciferase reporter assays performed by psiCHECK-2 vector. (B) RAD9A and MYOG mRNA expression after miR-2425-M transfection at 24 h, 48 h, and 72 h. (C) RAD9A protein expression after transfection of miR-2425-M or miR-2425-I. (D) Quantified data from (C). (E) MYOG protein expression after transfection of miR-2425-M or miR-2425-I. (F) Quantified data from (E). *P < 0.05, **P < 0.01, NS: no significant difference.

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