Table 2 Primers used for plasmid construction.

From: MiR-2425-5p targets RAD9A and MYOG to regulate the proliferation and differentiation of bovine skeletal muscle-derived satellite cells

gene

Forward primer

Reverse primer

pre-miR-2425

cggggtacctcccttctttacctgactt

ccggaattc cctttgttaccccgactt

psiCHECK-2-RAD9A-3′-UTR

ccctcgagggccctgctttgccctgagctgcag

atgcggccgcagcagcatccagtctccc

psiCHECK-2-RAD9A-3′-UTR -mut

ccctcgagggccctgctttcggctgagctgcagcctta

atgcggccgcagcagcatccagtctccc

psiCHECK-2-MYOG-3′-UTR

ccgctcgagatctgaccaaggtctctgtgctgaagttg

ataagaatgcggccgcctagcacccagtctttattt

psiCHECK-2-MYOG-3′-UTR -mut

ccgctcgagatctgaccaaggtctcacacgtgaagttgc

ataagaatgcggccgcctagcacccagtctttattt

miR-2425-intron of NCKAP5L

caggagaaggtggtgatgg

gataagtgagagaagccccagcagg

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