Figure 4

Reduced sensitivity of CaREL1-OX transgenic Arabidopsis lines to ABA. (a) Reverse transcription-polymerase chain reaction (RT-PCR) analysis of CaREL1 expression in wild-type and CaREL1-OX plants. Actin8 was used as an internal control gene. (b) Seed germination of wild-type and CaREL1-OX plants in response to ABA for 0–5 days after treatment. Seeds were germinated on 0.5× MS agar plates containing 0.0 μM, 0.5 μM, 0.75 μM, or 1.0 μM ABA. (c) Growth of wild-type and CaREL1-OX seedlings on 0.5× MS agar plates containing 0.0 μM, 0.5 μM, 0.75 μM, or 1.0 μM ABA. Representative photographs were taken 10 days after plating. (d) Quantification of green cotyledons in the wild-type and each mutant line was performed 10 days after plating on 0.5× MS agar plates containing 0.0 μM, 0.5 μM, 0.75 μM, or 1.0 μM ABA. Data represent the mean ± standard error of three independent experiments, each evaluating 30 seeds. (e) Root elongation of wild-type and transgenic plants in response to ABA. (f) The root length of each plant was measured 10 days after plating. Data represent the mean ± standard error of three independent experiments. Different letters indicate significant differences between wild-type and transgenic lines (P < 0.05; ANOVA followed by Fisher’s LSD test).