Figure 5
From: Pepper CaREL1, a ubiquitin E3 ligase, regulates drought tolerance via the ABA-signalling pathway
Reduced tolerance of CaREL1-OX plants to drought stress. (a) Drought tolerance of CaREL1-OX transgenic plants. Three-week-old wild-type and transgenic plants were subjected to drought stress by withholding water for 9 days and then re-watering for 2 days. Survival rates of plants were measured after re-watering. (b) Reduced biomass of CaREL1-OX plants. The plant biomass was measured after re-watering. (c) Transpirational water loss from the leaves of wild-type and transgenic plants at various times after detachment of leaves. (d and e) Decreased leaf temperatures of CaREL1-OX plants in response to ABA treatment. Leaf temperatures of plants treated with 50 μM ABA were measured using thermal imaging and representative images were taken (d). Data represent the mean ± standard error of three independent experiments (e). (f and g) Stomatal apertures in wild-type and CaREL1-OX plants treated with ABA. Leaf peels were harvested from 3-week-old plants of each line and incubated in SOS buffer containing 0 μM or 20 μM ABA. Stomatal apertures were measured under the microscope and representative images were taken (e). Data represent the mean ± standard error of three independent experiments (f), each evaluating 20 plants. Different letters indicate significant differences between wild-type and transgenic lines (P < 0.05; ANOVA followed by Fisher’s LSD test).
