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Figure 1

From: Pharmacokinetic-Pharmacodynamic modelling of intracellular Mycobacterium tuberculosis growth and kill rates is predictive of clinical treatment duration

Figure 1

Automated work flow of image analysis in Harmony. THP-1 macrophages infected with GFP-H37Rv were imaged on the Operetta platform described in the Methods section. The images were subsequently analysed with Harmony software in the following work flow (a) Image capture from one field of view displaying brightfield (grey), Hoechst (blue), and GFP-H37Rv (green). (b) Image without brightfield, displaying Hoechst (blue) and GFP-H37Rv (green). (c) Identification of extracellular GFP-H37Rv, detected M. tuberculosis are highlighted in red, the nucleus of the macrophages is in grey. (d) Identification of the nucleus of macrophages, detected nuclei (pink). (e) Cytoplasm detection, cytoplasm in blue and yellow. (f) M. tuberculosis infected macrophage (outline in orange), intracellular M. tuberculosis highlighted in red.

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