Figure 2
From: Identification of small molecule inhibitors of Interleukin-18
Competitive ELISA and IC50 determination. Dose-response curves with varying compound concentrations for the inhibition of hIL-18:ectvIL-18BP complex formation. IC50 values were obtained by nonlinear regression fitting to a variable slope, three-parameter dose-response model using the GraphPad Prism 6 software (San Diego, CA, USA). Competitive ELISA experiments were performed using four technical replicates. (A) Competitive ELISA curve for NSC201631, IC50 = 44+/− 9 uM, n = 3. (B) Competitive and Synergistic ELISA results for NSC80734, IC50 = 52 + / 38 uM, n = 3 and NSC201631/80734 (IC50 = 9.3 uM). (C) Competitive and Synergistic ELISA results for NSC61610, IC50 = 6+/− 5 uM, n = 2, and NSC201631/61610 (IC50 = 3.7 uM) (D) Two-Dimensional Chemical Structure of NSC201631, NSC61610, an NSC80734. Note: Synergistic competitive ELISA results obtained from four technical replicates of a single assay (n = 1). Standard deviation from mean is plotted with error bars.
