Figure 2 | Scientific Reports

Figure 2

From: A starvation-induced regulator, RovM, acts as a switch for planktonic/biofilm state transition in Yersinia pseudotuberculosis

Figure 2

Deletion of rovM causes aggregates. (a,b) Observation of cell-cell aggregates formed by wild-type strain, ΔrovM and ΔrovM(rovM) grown in M9 medium in tubes (a) and under SEM (b). Scale bar = 10 μm. (c) WT, ΔrovM and ΔrovM(rovM) grown to late exponential phase in M9 medium stained by Congo red. (d) Cell aggregates formed in ΔrovM culture were treated with (Right) or without (Left) sodium metaperiodate (1 M) at 4 °C for 24 h. Red arrow indicates aggregate adhered on tube. (e) Fluorescence microscopy image of ΔrovM aggregates stained by WGA-R. (f) Extracellular polysaccharide content in the Y. pseudotuberculosis WT, ΔrovM and ΔrovM(rovM) determined by the MBTH assay. Data shown are the average of three independent experiments; error bars indicate SD from three independent experiments. **P < 0.01.

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