Figure 6
Decreasing access to AHL can increase resistance but reduces cooperation. (A) To reduce access to AHL in the model, we decreased δ by 0.7 at 3/hr (relative to δ presented in Fig. 2E). Experimentally, we mixed the bacteria in the well at the beginning of the experiment. For all panels, squares indicate decreased δ. Circles represent data re-plotted from Fig. 4. (B) Simulations results (Equations (1–3)) showing P CRIT with decreased δ. For simplicity, we show the three shaking frequencies measured experimentally. (C) The result of individual simulations for each shaking frequency examined. For panels (C,E), green arrows indicate resistance increased (single ended arrow) or remained unchanged (double ended arrow). Direction of arrow indicates perturbation to P CRIT . (D) P CRIT of engineered bacteria that were initially well-mixed in the microplate well. Two-tailed t-tests for each P CRIT in Supplementary Tables S1 and S2, including comparisons between the well-mixed and non well-mixed initial conditions. SD from a minimum of three biological replicates. (E) Individual experiments for each agar density. Raw data in Supplementary Fig. S6.
