Figure 5

TRPV1 sensitization via 9-HODE-dependent activation of G2A is mediated by PKC but not PKA. (A) Sensitization of TRPV1 via 9-HODE (200 nM, 120 s) of adult DRG neurons is not influenced by the PKA-Inhibitor H89 (10 µM, 45 min before measurement) in calcium imaging experiments. As control for PKA-dependent TRPV1 sensitization 8-bromo-cAMP (40 µM, 120 s) was used. Data shown as mean ± SEM with n = 20–69 cells per condition. (B) The PKC-Inhibitor GF109203X (1 µM, 45 min before measurement) suppresses the 9-HODE-dependent sensitization of TRPV1. Bradykinin (500 nM, 120 s) was used as positive control for PKC-dependent sensitization of TRPV1. Data is shown as mean ± SEM with n = 45–90 cells per condition; *p < 0.05 **p < 0.01 one-way ANOVA with Bonferroni post-hoc test.