Table 1 Sequencing results of serially diluted DNA isolated from two adenocarcinoma cell lines (HT-29 y Caco-2) with known variants in BRAF and MC1R genes.
HT-29 in Caco-2 (variant frequency) | BRAF gene, p.V600E (heterozygous) | MC1R gene, p.R160W (heterozygous) | ||||||
|---|---|---|---|---|---|---|---|---|
Allele frequency (%) | Coverage (x) | Allele frequency (%) | Coverage (x) | |||||
Exp 1 | Exp 2 | Exp 1 | Exp 2 | Exp 1 | Exp 2 | Exp 1 | Exp 2 | |
Undiluted (50%) | 57.12 | 52.85 | 2027 | 2182 | 61.42 | 64.48 | 1894 | 2329 |
1:1 (25%) | 26.22 | 25.69 | 4366 | 5780 | 32.66 | 32.93 | 2103 | 2708 |
1:3 (12.5%) | 13.13 | 12.85 | 5620 | 3507 | 14.84 | 15.53 | 2182 | 2524 |
1:24 (2%) | 1.82 | 1.75 | 3720 | 3523 | 1.95 | 1.21 | 1894 | 1981 |
1:49 (1%) | 1.12 | 0.68 | 4266 | 3927 | 0.80 | 0.87 | 2967 | 2631 |
1:99 (0.5%) | 0.65 | 0.64 | 3977 | 3430 | 0.57 | 0.34 | 2624 | 1458 |
1:999 (0.05%) | 0.04 | 0.10 | 4993 | 2945 | 0.03 | 0.19 | 3065 | 3138 |
