Table 4 Turn-around time and cost comparison between Next-generation sequencing and conventional molecular analysis.
Analysis | System | Time duration (min) | Costs** (€) | |
|---|---|---|---|---|
NGS analysis (8 samples; 35 genes) | ||||
Quantification and sample dilution | Qubit | 60 | 4 | |
Library preparation | Veriti Thermal Cycler | 310 | 1819.8 | |
Emulsion PCR | One Touch | 315* | 238.8 | |
Enrichment | One Touch ES | 45 | 47.2 | |
Sequencing (two 318 chip v2) | PGM System | 360 (x2) | 1214 | |
Data processing and analysis | Ion Reporter | 110 | 0 | |
Laboratory personal costs | NA | 288 | ||
Total | 1,560 (=26 h) | 3323.8 | ||
Working days | 3 | NA | ||
Analysis | Method | |||
Conventional molecular analysis (8 samples; 3 genes) | ||||
Quantification | Nanodrop | 30 | 0 | |
BRAF | Exon 15 | RQ-PCR HRM+ SS | 510 (=81/2 h) | 460.2 |
NRAS | Exon 2/3/4 | RQ-PCR (hibridization probes)+ SS | 420 (=7 h) | 1,225.6 |
KIT | Exon 9/11/13/17 | Conventional PCR + SS | 1,230 (201/2 h) | 1,555.4 |
Laboratory personal costs | NA | 480 | ||
Total | 2,160 (=36 1/2 h) | 3,723.2 | ||
Working days | 5 | NA | ||
