Figure 3
pri-miR-9-BMSCs could inhibit the local inflammatory response. The expressions of pro-inflammatory cytokines(IL-1β, IL-6, TNF-α, and MPO), NF-κB proteins (p-P65, P50) and CD68 were significantly decreased by pir-miR-9-BMSCs. On the contrary, the expressions of superoxide dismutase (SOD1 and SOD2) and anti-inflammatory proteins (IκBα and IκBβ) were significantly increased by pir-miR-9-BMSCs, compared with SAP, SAP+PBS, BMSCs, or TuD-BMSCs groups by gPCR (A,B), Western-blot (C,D) and IHC (E,F). Data are shown as mean ± SD for at least 3 separate experiments. %%P < 0.01, compared with NC, #p < 0.05, ##p < 0.01 and ###p < 0.001, compared with NC, +p < 0.05, ++p < 0.01 and +++p < 0.001, compared with SAP, *p < 0.05 and **p < 0.01, compared with SAP+PBS, @p < 0.05 and @@p < 0.01, compared with BMSCs, $p < 0.05 and $$p < 0.01, compared with Empty-virus BMSCs, †p < 0.05, ††p < 0.01 and †††p < 0.001, compared with TuD-BMSCs by using paired t test. gPCR, General PCR, IHC, immunohistochemistry.
