Figure 3
Location of identified mutations. (a) Illustration of the location of identified mutations screened with temperature (top panel) or capsaicin (bottom panel) for ‘functional’ clones (blue and yellow), ‘less functional’ clones (gray) or all identified mutations pooled together (purple). In yellow are ‘functional’ mutations that conserved wild-type amino acid hydropathy. In blue are ‘functional’ mutations that did not conserve amino acid hydropathy. Mutations were considered hydropathy-conserving if the Δhydropathy (hydropathymutant − hydropathyWT) = ±0.6. Mutations outside of that range are considered non-conserving. Each bar indicates one single mutation and boxes on top illustrate the structural domains of TRPV1. (b) Histogram of frequencies of gap lengths between mutations of temperature (top) or capsaicin (bottom) ‘functional’ category and gap lengths within ten random distributions of 287 (temperature, top) or 248 (capsaicin, bottom) mutations in 838 positions. Error bars are SEM.
