Figure 4
From: Cancer-associated noncoding mutations affect RNA G-quadruplex-mediated regulation of gene expression
Further characterization of the RNA G4 destabilising mutation in the 5′ UTR of BCL2 (A) CD spectra of wild-type and mutated BCL2 oligomer which consist of 22 nucleotides predicted to form the G4 and 20 flanking nucleotides in KCl. (B) CD melting curves of wild-type and mutated BCL2 oligomer at 260 nm. (C) Gene expression level of wild-type and G4-mutated construct at the protein level as determined by dual luciferase assay and the mRNA level as determined by RT-qPCR. Data were analysed using multiple t-test (n ≥ 2, error bars represent the SEM, asterisks (*) indicate significance and ns indicates non-significant).
