Table 1 Melting temperatures of RNA G4 oligos annealed in 100 mM KCl, 20 mM Tris-HCl, pH 7.5.

From: Cancer-associated noncoding mutations affect RNA G-quadruplex-mediated regulation of gene expression

RNA G4 oligos

T m (°C)a

BCL2

wt

78 ± 2.1

mut

64 ± 0.2

Long BCL2 b

wt

74.5 ± 3.5

mut

58 ± 2

CXCL14

wt

>90c

mut

73 ± 0.5

TAOK2

wt

68 ± 0.5

mut

75 ± 1

  1. a T m values are expressed as mean ± SD (n ≥ 2).
  2. bLong BCL2 consists of 22 nucleotides predicted to form the RNA G4 and 20 extra nucleotides from each side.
  3. c CXCL14 wt RNA G4 oligo could not be completely melted even at 95 °C.