Figure 3
From: The 5′UTR in human adenoviruses: leader diversity in late gene expression
The putative i-leader protein terminates in the TPL3 of HAdV-D37. (A) Nucleotide sequence, (B) RT-PCR gel photomicrograph, and (C) schematic for i-leader spliced to TPL3 of 5′UTR for fiber, and in the putative i-protein mRNA, in which the 5′UTR is TPL1-2. RT-PCR as shown was performed with forward primer from TPL1 and reverse primers either from within fiber gene or the end of the predicted i-protein. Sequencing of gel purified transcripts revealed two splice variants of the putative i-leader mRNA, as shown. The putative i-protein mRNA is preceded by a 26 nucleotide 5′UTR prior to the start site (yellow) of an ORF (7786) for the potential coding region that would terminate (red) either within TPL3 (as shown for fiber gene), or at nucleotide 8253, the latter coding for the putative i-protein.
